1V2A

Glutathione S-transferase 1-6 from Anopheles dirus species B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2001-07-25
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	48.203, 88.136, 200.446
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.150
Rwork	0.234
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jlv
RMSD bond length	0.006
RMSD bond angle	20.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.106	0.405
Number of reflections	43837
<I/σ(I)>	9.56	2
Completeness [%]	91.9	90.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	22.5% PEG 4000, 0.2M ammonium acetate, 0.1M sodium acetate/acetic acid pH 5.0, 10mM glutathione sulfonic acid, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		1.6-2 (M)
2	1	reservoir		1 (mM)
3	1	reservoir	sodium phosphate	100 (mM)	pH6.5-7.5
4	1	drop	protein	13.1 (mg/ml)
5	1	drop	glutathione sulphonic acid	10 (mM)