1V2A
Glutathione S-transferase 1-6 from Anopheles dirus species B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2001-07-25 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.203, 88.136, 200.446 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.150 |
Rwork | 0.234 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jlv |
RMSD bond length | 0.006 |
RMSD bond angle | 20.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.106 | 0.405 |
Number of reflections | 43837 | |
<I/σ(I)> | 9.56 | 2 |
Completeness [%] | 91.9 | 90.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 22.5% PEG 4000, 0.2M ammonium acetate, 0.1M sodium acetate/acetic acid pH 5.0, 10mM glutathione sulfonic acid, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 1.6-2 (M) | ||
2 | 1 | reservoir | 1 (mM) | ||
3 | 1 | reservoir | sodium phosphate | 100 (mM) | pH6.5-7.5 |
4 | 1 | drop | protein | 13.1 (mg/ml) | |
5 | 1 | drop | glutathione sulphonic acid | 10 (mM) |