1V26

Crystal structure of tt0168 from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-02-19
Detector	RIGAKU RAXIS V
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	64.510, 101.381, 176.898
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.100 - 2.500
R-factor	0.20743
Rwork	0.204
R-free	0.24046
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.923
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.100	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	41062
Completeness [%]	99.8	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	MES, ammonium sulfate, PEG-MME , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K