1V1T
Crystal structure of the PDZ tandem of human syntenin in complex with TNEYKV peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-04 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 72.341, 72.341, 125.676 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.250 - 1.800 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n99 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.475 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.091 | 0.345 |
Number of reflections | 34079 | |
<I/σ(I)> | 19 | 5.7 |
Completeness [%] | 98.7 | 99.8 |
Redundancy | 7 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 20% PEG3350, 0.2M NH4CL, VAPOR DIFFUSION, SITTING DROP, pH 7.50 |