1V1Q

Crystal structure of PriB- a primosomal DNA replication protein of Escherichia coli

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL17B2
Synchrotron site	NSRRC
Beamline	BL17B2
Temperature [K]	110
Detector technology	IMAGE PLATE
Detector	RIGAKU IMAGE PLATE
Wavelength(s)	1.0083, 1.0093, 0.9921, 1.0249
Spacegroup name	P 21 21 21
Unit cell lengths	39.322, 57.358, 97.019
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.170 - 2.100
R-factor	0.206
Rwork	0.206
R-free	0.26300
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.170	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.083	0.561
Number of reflections	13751
<I/σ(I)>	14.1	2.2
Completeness [%]	99.9	99.6
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.7		pH 5.70