1V1P

The structure SSL from Staphylococcus Aureus from an orthorhombic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-15
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	51.653, 71.598, 103.471
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.722 - 2.700
R-factor	0.23526
Rwork	0.232
R-free	0.30070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m4v
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.010	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.140	0.490
Number of reflections	11074
<I/σ(I)>	4.5902	1.53
Completeness [%]	99.9	99.9
Redundancy	4.5	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		0.1M TRICINE BUFFER @PH 8.5, 28% (W/V) PEG2K, 0.1M LITHIUM SULPHATE