1V1O
staphylococcal superantigen-like protein 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.660, 81.660, 148.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.360 - 2.750 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.25900 |
| Structure solution method | MAD |
| Starting model (for MR) | 1m4v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.091 | 0.460 |
| Number of reflections | 59998 | |
| <I/σ(I)> | 14.5 | 3.1 |
| Completeness [%] | 98.7 | 99.2 |
| Redundancy | 4.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 28% PEG-MME 2K, 0.2 M AMMONIUM SULPHATE, 0.1 M MES, PH 6.5 |
