1V1O

staphylococcal superantigen-like protein 7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-15
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	81.660, 81.660, 148.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.360 - 2.750
R-factor	0.213
Rwork	0.213
R-free	0.25900
Structure solution method	MAD
Starting model (for MR)	1m4v
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.500	2.900
High resolution limit [Å]	2.750	2.750
Rmerge	0.091	0.460
Number of reflections	59998
<I/σ(I)>	14.5	3.1
Completeness [%]	98.7	99.2
Redundancy	4.5	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		28% PEG-MME 2K, 0.2 M AMMONIUM SULPHATE, 0.1 M MES, PH 6.5