1V1G
Structure of the Arabidopsis thaliana SOS3 complexed with Calcium(II) ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 100 |
Collection date | 2003-10-15 |
Spacegroup name | I 41 |
Unit cell lengths | 91.600, 91.600, 85.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.237 |
Rwork | 0.235 |
R-free | 0.26400 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.735 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.000 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.057 | 0.540 |
Number of reflections | 9757 | |
<I/σ(I)> | 4.4 | 1.4 |
Completeness [%] | 100.0 | 99.6 |
Redundancy | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.8 | PRIOR TO CRYSTALLIZATION THE PROTEIN WAS PREINCUBATED WITH 3.8 MM CACL2 TO REACH A FINAL PROTEIN CONCENTRATION OF 10 MG/ML AND A PRTEIN:METAL ION RATIO 1:10. CRYSTALLIZATION EXPERIMENTS WERE CARRIED OUT AT ROOM TEMPERATURE. CRYSTALS WERE GROWN USING VAPOUR DIFFUSION TECHNIQUES FROM DROPS CONTAINING SOS3-CA, RESERVOIR SOLUTIONS (24%(W/V) METHYL-PENTANEDIOL, 18%(W/V) POLYETHYLENE GLYCOL 4000 AND 0.1 NA-CITRATE PH 4.8) AND NAI 1M IN A RATIO 2:1:0.75 |