1V10

Structure of Rigidoporus lignosus laccase from hemihedrally twinned crystals

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Spacegroup name	P 65
Unit cell lengths	124.914, 124.914, 95.117
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.226
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gyc
RMSD bond length	0.008
RMSD bond angle	0.050
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.700
Rmerge	0.057
Number of reflections	91011
Completeness [%]	99.0
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		pH 6.50