1V0Y
Phospholipase D from Streptomyces sp. strain PMF soaked with the substrate dibutyrylphosphatidylcholine.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM1A |
Synchrotron site | ESRF |
Beamline | BM1A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.369, 56.462, 68.762 |
Unit cell angles | 90.00, 93.74, 90.00 |
Refinement procedure
Resolution | 13.710 - 1.710 |
R-factor | 0.162 |
Rwork | 0.160 |
R-free | 0.20200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | BASED ON PDB ENTRY 1F0I |
RMSD bond length | 0.019 |
RMSD bond angle | 1.972 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.710 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.115 | 0.473 |
Number of reflections | 45138 | |
<I/σ(I)> | 4.5 | 2 |
Completeness [%] | 99.2 | 92.9 |
Redundancy | 4.9 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.4 | 0.2 M NH4AC, 0.1 M CITRATE PHOSPHATE BUFFER AT PH5.4, 27.5% PEG 4000. CRYSTALS WERE THEREAFTER BACKSOAKED TWICE IN A PHOSPHATE- FREE BUFFER TO REMOVE TRACE-AMOUNTS OF PHOSPHATE., pH 5.40 |