1V0R
Tungstate-inhibited phospholipase D from Streptomyces sp. strain PMF
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.610, 56.900, 68.950 |
| Unit cell angles | 90.00, 93.67, 90.00 |
Refinement procedure
| Resolution | 69.010 - 1.700 |
| R-factor | 0.157 |
| Rwork | 0.155 |
| R-free | 0.19800 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.992 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.010 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.043 | 0.250 |
| Number of reflections | 46471 | |
| <I/σ(I)> | 11.8 | 3 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.4 | 0.2 M NH4AC, 0.1 M CITRATE PHOSPHATE BUFFER AT PH5.4, 27.5% PEG 4000, pH 5.40 |
