1V0N
Xylanase Xyn10a from Streptomyces lividans in complex with xylobio-isofagomine at pH 7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.328, 46.132, 86.382 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.850 - 1.100 |
| R-factor | 0.104 |
| Rwork | 0.103 |
| R-free | 0.12400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1od8 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.730 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.056 | 0.217 |
| Number of reflections | 97030 | |
| <I/σ(I)> | 15.6 | 4.6 |
| Completeness [%] | 95.1 | 70.4 |
| Redundancy | 5.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM IMIDAZOLE, PH 7.5 16% PEG 5K MME, 5% ISOPROPANOL |
