1V0L
Xylanase Xyn10A from Streptomyces lividans in complex with xylobio-isofagomine at pH 5.8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.018, 46.098, 86.254 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.820 - 0.980 |
| R-factor | 0.119 |
| Rwork | 0.118 |
| R-free | 0.13700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1od8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.727 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.020 |
| High resolution limit [Å] | 0.980 | 0.980 |
| Rmerge | 0.058 | 0.406 |
| Number of reflections | 140638 | |
| <I/σ(I)> | 26.7 | 4.23 |
| Completeness [%] | 93.4 | 89.1 |
| Redundancy | 6.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 20 MG/ML PROTEIN, 0.1 M MES 18% PEG 5KMME, 7.5% ISOPROPANOL, pH 5.80 |
