1V0J
Udp-galactopyranose mutase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2002-07-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 137.601, 153.733, 137.688 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.250 |
| R-factor | 0.227 |
| Rwork | 0.225 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i8t |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.543 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0007) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.500 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.100 | 0.610 |
| Number of reflections | 1188969 | |
| <I/σ(I)> | 3.3 | 1.1 |
| Completeness [%] | 89.0 | 85.2 |
| Redundancy | 6.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 0.1M BICINE PH9.0, 1.6M AMMONIUM SULPHATE, 277K, pH 9.00 |
