1V0B

Crystal structure of the t198a mutant of pfpk5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	55.290, 94.653, 133.229
Unit cell angles	90.00, 99.19, 90.00

Refinement procedure

Resolution	129.100 - 2.200
R-factor	0.226
Rwork	0.223
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.410
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	27.000
High resolution limit [Å]	2.200
Rmerge	0.098
Number of reflections	33495
Completeness [%]	97.0
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4		pH 7.40