1V0A
Family 11 Carbohydrate-Binding Module of cellulosomal cellulase Lic26A-Cel5E of Clostridium thermocellum
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 110 |
Wavelength(s) | 0.981033,0.981266, 0.976315 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 75.143, 50.892, 40.868 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.000 - 1.980 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.23200 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.829 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.299 | 2.020 |
High resolution limit [Å] | 1.977 | 1.980 |
Rmerge | 0.059 | 0.099 |
Number of reflections | 11449 | |
<I/σ(I)> | 30.3 | 8.4 |
Completeness [%] | 99.5 | 99.3 |
Redundancy | 1.84 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | pH 6.00 |