1V08
Crystal structure of the Zea maze beta-glucosidase-1 in complex with gluco-tetrazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Collection date | 2001-02-15 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.180, 104.800, 119.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.750 - 1.900 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e1e |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.189 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 2.010 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.072 | 0.456 |
| Number of reflections | 92037 | |
| <I/σ(I)> | 6.9 | 1.5 |
| Completeness [%] | 96.6 | 94 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 22% PEG 4000, 5% ISOPROPANOL, 0.1 M HEPES PH 7.5 |
