1V02
Crystal structure of the Sorghum bicolor dhurrinase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Collection date | 2003-01-15 |
| Spacegroup name | P 31 |
| Unit cell lengths | 101.050, 101.050, 279.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e1e |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.336 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.068 | 0.339 |
| Number of reflections | 291187 | |
| <I/σ(I)> | 8 | 2.1 |
| Completeness [%] | 98.6 | 89.6 |
| Redundancy | 4.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 16% PEG-MONO-METHYL-ETHER 2000, 0.1 M TRIS PH 7.0 |
