1UZZ
Erythrina cristagalli bound to N-linked oligosaccharide and lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2002-02-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.280, 55.370, 86.930 |
| Unit cell angles | 86.23, 75.37, 82.13 |
Refinement procedure
| Resolution | 40.000 - 2.130 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.210 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.070 | 0.200 |
| Number of reflections | 55200 | |
| <I/σ(I)> | 10.9 | 5.2 |
| Completeness [%] | 97.6 | 86.1 |
| Redundancy | 1.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 17% PEG 3350, 0.3M SODIUM CHLORIDE,0.02M IMIDAZOLE, pH 7.00 |
