1UZX
A complex of the Vps23 UEV with ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.934, 66.032, 69.333 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.690 - 1.850 |
R-factor | 0.241 |
Rwork | 0.241 |
R-free | 0.27400 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.066 | 0.320 |
Number of reflections | 23553 | |
<I/σ(I)> | 22.7 | 2.36 |
Completeness [%] | 99.3 | 97.2 |
Redundancy | 9.07 | 3.71 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 22% PEG 8000, 0.1M MES PH 6.5, 0.2M (NH4)2SO4 |