1UZV
High affinity fucose binding of Pseudomonas aeruginosa lectin II: 1.0 A crystal structure of the complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.735, 72.827, 54.747 |
| Unit cell angles | 90.00, 94.21, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.000 |
| R-factor | 0.1146 |
| R-free | 0.13510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PSEUDOMONAS AERUGINOSA LECTIN |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.029 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.880 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.063 | 0.230 |
| Number of reflections | 216831 | |
| <I/σ(I)> | 5 | 2.8 |
| Completeness [%] | 97.7 | 94.3 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | TRIS HCL 0.1M, PH8.5, 1.75 M AMMONIUM SULFATE, pH 8.50 |
