1UZJ
Integrin binding cbEGF22-TB4-cbEGF33 fragment of human fibrillin-1, holo form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.100, 105.600, 65.000 |
| Unit cell angles | 90.00, 104.20, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.250 |
| R-factor | 0.247 |
| Rwork | 0.247 |
| R-free | 0.31800 |
| Structure solution method | MAD |
| Starting model (for MR) | PBD FIBRILLIN I222 FORM |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.540 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.099 | 0.450 |
| Number of reflections | 25753 | |
| <I/σ(I)> | 9.7 | 2.2 |
| Completeness [%] | 96.2 | 92.6 |
| Redundancy | 2.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 5MG/ML PROTEIN IN 10MM MES 10MM CACL2 PH6. DEHYDRATED AGAINST: 15% PEG 8000, 10MM MES PH 6 10MM CACL2 |
