1UZH

A CHIMERIC CHLAMYDOMONAS, SYNECHOCOCCUS RUBISCO ENZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-15
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	220.820, 223.969, 111.752
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.162
Rwork	0.160
R-free	0.19300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gk8
RMSD bond length	0.013
RMSD bond angle	1.235
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.148	0.444
Number of reflections	277345
<I/σ(I)>	8.65	2
Completeness [%]	90.2	81.6
Redundancy	20.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	291	50 MM HEPES PH 7.5, 8-12% PEG 4 50 MM NAHCO3, 5 MM MGCL2, 50 UM 2-CABP, 18 DEG C, 10-15 MG PROTEIN