1UZB
1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-15 |
| Detector | RIGAKU RAXIS-V |
| Spacegroup name | H 3 |
| Unit cell lengths | 102.285, 102.285, 279.283 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 * - 1.400 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bi9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.700 * |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.045 | 0.181 |
| Number of reflections | 211697 | |
| <I/σ(I)> | 38.99 | 7.75 |
| Completeness [%] | 98.6 | 97.1 |
| Redundancy | 3.51 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 298 * | Inagaki, E., (2005) Acta Cryst., F61, 609. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 32 (%) | |
| 3 | 1 | reservoir | sodium citrate | 50 (mM) | pH5.2 |
