1UZ9
Crystallographic and solution studies of N-lithocholyl insulin: a new generation of prolonged-acting insulins.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 51.790, 51.790, 68.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.000 - 1.600 |
| R-factor | 0.18 |
| Rwork | 0.179 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xda |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.821 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.034 | 0.139 |
| Number of reflections | 7689 | |
| <I/σ(I)> | 33 | 14 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 5.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.5 M TRIS-HCL PH 8.0 0.1M TRI-SODIUM CITRATE, 2MM ZINC ACETATE, 0.05% W/V M-CRESOL |
