1UZ8
anti-Lewis X Fab fragment in complex with Lewis X
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-25 |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 45.104, 60.778, 91.556 |
Unit cell angles | 95.98, 95.41, 101.71 |
Refinement procedure
Resolution | 91.290 - 1.800 |
Rwork | 0.210 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uz6 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.151 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.175 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.094 | 0.386 |
Number of reflections | 81582 | |
<I/σ(I)> | 11.1 | 2.2 |
Completeness [%] | 92.6 | 72 |
Redundancy | 4.1 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 14 % PEG 3350, 50 MM TRIS PH 8 |