1UZ6
anti-Lewis X Fab fragment uncomplexed
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-25 |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 67.405, 71.559, 104.790 |
Unit cell angles | 86.48, 71.32, 83.26 |
Refinement procedure
Resolution | 45.180 - 2.050 |
Rwork | 0.198 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1clz |
RMSD bond length | 0.014 |
RMSD bond angle | 1.144 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.175 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.046 | 0.329 |
Number of reflections | 102864 | |
<I/σ(I)> | 19.7 | 2.1 |
Completeness [%] | 88.1 | 38.9 |
Redundancy | 2.5 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 26 % PEG 3350, 0.17 M AMMONIUM SULFATE, 15 % GLYCEROL, pH 8.00 |