1UZ1
Family 1 b-glucosidase from Thermotoga maritima in complex with isofagomine lactam
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.412, 94.327, 113.063 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.550 - 2.000 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1od0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.303 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 | 0.437 |
| Number of reflections | 65765 | |
| <I/σ(I)> | 24.3 | 2.34 |
| Completeness [%] | 96.0 | 84.5 |
| Redundancy | 4.95 | 2.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10MG/ML PROTEIN IN 15% PEG 4000, 0.2 M CAAC, 0.1 M IMIDAZOLE PH7, pH 7.00 |
