1UYV

Acetyl-CoA carboxylase carboxyltransferase domain L1705I/V1967I mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Spacegroup name	C 1 2 1
Unit cell lengths	247.170, 123.730, 145.640
Unit cell angles	90.00, 94.20, 90.00

Refinement procedure

Resolution	28.000 * - 2.600
R-factor	0.212
Rwork	0.212
R-free	0.23700
Structure solution method	OTHER
RMSD bond length	0.007
RMSD bond angle	22.800 *
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.050	0.256
Total number of observations	315564 *
Number of reflections	117132
<I/σ(I)>	16	3
Completeness [%]	95.0	89
Redundancy	2.3	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.5		pH 5.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	0.1 (M)	pH5.5
2	1	reservoir		200 (mM)
3	1	reservoir	PEG8000	8 (%(w/v))
4	1	reservoir	glycerol	10 (%(v/v))
5	1	drop	protein	10 (mg/ml)