1UYU
Xenon COMPLEX OF wildtype P450CAM FROM PSEUDOMONAS PUTIDA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF |
Synchrotron site | ESRF |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.200, 62.300, 95.300 |
Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
Resolution | 19.840 - 2.000 |
R-factor | 0.19 |
Rwork | 0.187 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dz8 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.421 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.121 | 0.483 |
Number of reflections | 53282 | |
<I/σ(I)> | 9.3 | 4.2 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 3.82 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 275 | CRYSTALS WERE GROWN USING THE SITTING DROP GEOMETRY AT 2 DEG. C. 5 UL OF 30 MG/ML P450 IN 50 MM TRIS HCL, 250 MM KCL, 0.5 MM CAMPHOR WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION (27-30% PEG 4000, 100 MM DTE, SAME BUFFER AS PROTEIN)., pH 7.40 |