1UY0

Carbohydrate binding module (CBM6cm-2) from Cellvibrio mixtus lichenase 5A in complex with glc-1,3-glc-1,4-glc-1,3-glc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Collection date	2003-10-15
Spacegroup name	P 21 21 21
Unit cell lengths	63.550, 67.650, 85.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.700 - 1.650
R-factor	0.155
Rwork	0.154
R-free	0.17700
Structure solution method	OTHER
RMSD bond length	0.013
RMSD bond angle	1.374
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	FFT
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.700	1.700
High resolution limit [Å]	1.650	1.650
Rmerge	0.033	0.340
Number of reflections	44886
<I/σ(I)>	13.9	2.2
Completeness [%]	99.9	99.9
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		11% PEG 6000, 2.0 M NACL, pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	PEG6000	12 (%(w/v))
3	1	reservoir		2.2 (M)