1UXZ
Carbohydrate binding module (CBM6cm-2) from Cellvibrio mixtus lichenase 5A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Collection date | 2002-06-15 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.110, 66.956, 85.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.520 - 1.400 |
R-factor | 0.162 |
Rwork | 0.161 |
R-free | 0.17700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nae |
RMSD bond length | 0.010 |
RMSD bond angle | 1.323 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.055 | 0.189 |
Number of reflections | 70856 | |
<I/σ(I)> | 9.1 | 3.2 |
Completeness [%] | 98.8 | 97.7 |
Redundancy | 4.9 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 11% PEG 6000, 2.0 M NACL, pH 7.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | reservoir | PEG6000 | 12 (%(w/v)) | |
3 | 1 | reservoir | 2.2 (M) |