1UX8
X-ray structure of truncated oxygen-avid haemoglobin from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 41.193, 41.193, 134.537 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.500 - 2.150 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ngk |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 2.150 |
Rmerge | 0.098 |
Number of reflections | 6975 |
Completeness [%] | 98.6 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 2,0 M AMMONIUM SULFATE, 5% V/V ISOPROPANOL, PH=6.0, pH 6.00 |