1UX5
Crystal Structures of a Formin Homology-2 domain reveal a flexibly tethered dimer architecture
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Wavelength(s) | 0.916, 0.936 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 101.400, 101.400, 265.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.25100 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.210 |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.500 | 2.500 * |
Rmerge | 0.087 | 0.458 * |
Total number of observations | 154481 * | |
Number of reflections | 49995 | |
<I/σ(I)> | 12.9 | |
Completeness [%] | 94.5 * | 90 * |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20 * | HANGING DROP AGAINST A WELL CONTAINING 16% ETHYLENE GLYCOL, pH 7.50 |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20 * | HANGING DROP AGAINST A WELL CONTAINING 16% ETHYLENE GLYCOL, pH 7.50 |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20 * | HANGING DROP AGAINST A WELL CONTAINING 16% ETHYLENE GLYCOL, pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | PCMBS | 2.5 (mM) | |
3 | 1 | reservoir | ethylene glycol | 16 (%) |