1UX1
Bacillus subtilis cytidine deaminase with a Cys53His and an Arg56Gln substitution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2002-09-23 |
Detector | MARRESEARCH |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 62.484, 62.484, 221.604 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.660 - 2.360 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jtk |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.410 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.059 | 0.217 |
Number of reflections | 21401 | |
<I/σ(I)> | 29.8 | 6.9 |
Completeness [%] | 98.9 | 83.9 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | VAPOUR DIFFUSION AT RT: PROTEIN: 4.6 MG/ML, + 5 MM TETRAHYDRODEOXYURIDINE, PRECIPITANT: 30 % PEG400, 0.2M MAGNESIUM CHLORIDE, 0.1 M HEPES PH 7.5 |