1UWW
X-ray crystal structure of a non-crystalline cellulose specific carbohydrate-binding module: CBM28.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.866, 65.926, 103.633 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.900 - 1.400 |
| R-factor | 0.19 |
| Rwork | 0.189 |
| R-free | 0.20800 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.502 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.926 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.094 | 0.325 |
| Number of reflections | 86838 | |
| <I/σ(I)> | 14.4 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 25% (W/V) MONOMETHYL ETHER PEG 2000, 200MM CA-ACETATE, 100MM NA-ACETATE PH 4.6 |
