1UWQ
Structure of beta-glycosidase from Sulfolobus solfataricus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-15 |
Detector | ADSC CCD |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 168.026, 168.026, 94.605 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 141.420 - 2.020 |
R-factor | 0.196 |
Rwork | 0.194 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gow |
RMSD bond length | 0.014 |
RMSD bond angle | 1.285 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.090 |
High resolution limit [Å] | 2.020 | 2.020 |
Rmerge | 0.069 | 0.461 |
Number of reflections | 99428 | |
<I/σ(I)> | 17.3 | 3.15 |
Completeness [%] | 99.0 | 98.7 |
Redundancy | 4.89 | 4.44 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 11-14% PEG 4K 0.1 M NA ACETATE, 0.2 M AMMONIUM ACETATE CRYO - 25% ETHYLENE GLYCOL, 10-13 MG/ML PROTEIN, pH 4.60 |