1UWP
Initial Events in the Photocycle of Photoactive Yellow Protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 85 |
| Detector technology | CCD |
| Collection date | 2002-03-30 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9393,0.9796 |
| Spacegroup name | P 63 |
| Unit cell lengths | 65.922, 65.922, 40.584 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.200 |
| R-factor | 0.137 |
| Rwork | 0.136 |
| R-free | 0.15700 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.979 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.230 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.035 | 0.199 |
| Number of reflections | 146753 | |
| <I/σ(I)> | 14.6 | 4.5 |
| Completeness [%] | 98.3 | 94.4 |
| Redundancy | 4.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.25 M NH4SO4, 20 MM SODIUM PHOSPHATE PH 7, VAPOR DIFFUSION, HANGING DROP, TEMP 298K |
