1UWF
1.7 A resolution structure of the receptor binding domain of the FimH adhesin from uropathogenic E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-15 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.906, 41.636, 97.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.270 - 1.690 |
| R-factor | 0.1791 |
| Rwork | 0.179 |
| R-free | 0.21810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qun |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.730 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.052 | 0.196 |
| Number of reflections | 18597 | |
| <I/σ(I)> | 27.25 | 8.2 |
| Completeness [%] | 98.8 | 96.8 |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.1 MM PROTEIN, 0.1 M HEPES PH 7.5, 10 % PEG6000, 5% V/V MPD |
