1UWC

Feruloyl esterase from Aspergillus niger

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	38.759, 40.182, 77.953
Unit cell angles	74.96, 78.63, 71.10

Refinement procedure

Resolution	74.540 - 1.080
R-factor	0.116
Rwork	0.115
R-free	0.13802
Structure solution method	DIRECT METHODS
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	ACORN
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.120
High resolution limit [Å]	1.080	1.080
Rmerge	0.058	0.260
Number of reflections	170583
<I/σ(I)>	15.8	2.5
Completeness [%]	93.5	82
Redundancy	2.9	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5		1.0M AMMONIUM SULPHATE, 0.1M NA ACETATE PH 4.5