1UWB

TYR 181 CYS HIV-1 RT/8-CL TIBO

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Detector technology	CCD
Collection date	1996-03
Spacegroup name	C 1 2 1
Unit cell lengths	225.700, 69.200, 104.900
Unit cell angles	90.00, 106.60, 90.00

Refinement procedure

Resolution	15.000 * - 3.200
R-factor	0.274
Rwork	0.274
R-free	0.36000
RMSD bond length	0.011
RMSD bond angle	23.700 *
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	3.200 *
Rmerge	0.105
Total number of observations	61059 *
Number of reflections	23319 *
Completeness [%]	90.0 *
Redundancy	2.62

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *	4 *	Clark, A.D., (1995) Methods Enzymol., 262, 171. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	19-20 (mg/ml)
2	1	drop	beta-OG	0.1 (%)
3	1	drop	DMSO	1.6 (%)
4	1	reservoir	Bis-Tris propane	50 (mM)
5	1	reservoir	ammonium sulfate	100 (mM)
6	1	reservoir	glycerol	10 (%)