1UW9
L290F-A222T chlamydomonas Rubisco mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Collection date | 2001-02-23 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 126.073, 178.196, 120.463 |
Unit cell angles | 90.00, 120.35, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.050 |
R-factor | 0.1569 |
Rwork | 0.157 |
R-free | 0.19421 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gk8 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.150 | 0.490 |
Number of reflections | 295370 | |
<I/σ(I)> | 8.9 | 1.8 |
Completeness [%] | 88.9 | 83.5 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 291 | 0 MM HEPES PH 7.5, 8-12% PEG 4 50 MM NAHCO3, 5 MM MGCL2, 50 UM 2-CABP, 18 DEG C, 10-15 MG PROTEIN |