1UVZ
structure of human thioredoxin 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-07-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.038, 49.087, 78.977 |
| Unit cell angles | 87.96, 83.19, 79.08 |
Refinement procedure
| Resolution | 23.250 - 2.010 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ert |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.017 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BEAST |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.500 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.220 |
| Number of reflections | 47020 | |
| <I/σ(I)> | 9.72 | 4.22 |
| Completeness [%] | 96.9 | 90.4 |
| Redundancy | 2.11 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | PEG 3350 22% (W/V), NA ACETATE 0.1M (PH: 4.6), AMMONIUM SULFATE 0.2M, DTT 1MM, pH 4.60 |
