1UVS
BOVINE THROMBIN--BM51.1011 COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 283 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 81.684, 81.684, 186.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.28000 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 24.300 * |
| Data reduction software | PROTEIN |
| Data scaling software | SAINT |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.079 |
| Number of reflections | 7921 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.9 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | thrombin | 10 (mg/ml) | |
| 10 | 1 | reservoir | PEG4000 | 30 (%) | |
| 2 | 1 | drop | citrate | 25 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | DMSO | 10 (mM) | |
| 5 | 1 | drop | lithium sulfate | 0.2 (M) | |
| 6 | 1 | drop | Tris | 0.1 (M) | |
| 7 | 1 | drop | PEG4000 | 30 (%) | |
| 8 | 1 | reservoir | lithium sulfate | 0.2 (M) | |
| 9 | 1 | reservoir | Tris | 0.1 (M) |
