1UVL

The structural basis for RNA specificity and Ca2 inhibition of an RNA-dependent RNA polymerase phi6p2 with 5nt RNA. Conformation B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	150
Collection date	2001-10-15
Spacegroup name	P 1 21 1
Unit cell lengths	105.946, 91.881, 140.762
Unit cell angles	90.00, 101.56, 90.00

Refinement procedure

Resolution	20.000 * - 2.000
R-factor	0.281
Rwork	0.281
R-free	0.29900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hhs
RMSD bond length	0.024
RMSD bond angle	23.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.066	0.732
Total number of observations	2110477 *
Number of reflections	176955 *
<I/σ(I)>	20.9	1.8
Completeness [%]	99.2	93.5
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.7 *	293 *	Butcher, S.J., (2000) Acta Crystallogr.,Sect.D, D56, 1473. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	PEG8000	10 (%)
3	1	reservoir	MES	0.1 (M)	pH6.7