1UVI

The structural basis for RNA specificity and Ca2 inhibition of an RNA-dependent RNA polymerase phi6p2 with 6nt RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	150
Detector technology	CCD
Collection date	2002-01-15
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	105.130, 93.711, 140.741
Unit cell angles	90.00, 101.22, 90.00

Refinement procedure

Resolution	20.000 * - 2.200 *
R-factor	0.23
Rwork	0.230
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hhs
RMSD bond length	0.011
RMSD bond angle	23.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.150
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.126	0.818
Total number of observations	1522219 *
Number of reflections	142686 *
<I/σ(I)>	13.3	1.6
Completeness [%]	97.8	83.7
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	7.3		Butcher, S.J., (2000) Acta Crystallogr.,Sect.D, D56, 1473. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	PEG8000	10 (%)
3	1	reservoir	MES	0.1 (M)	pH6.7