1UVH
X-ray structure of Dps from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Collection date | 2003-06-15 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 124.300, 124.300, 304.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.800 |
| R-factor | 0.27 |
| Rwork | 0.260 |
| R-free | 0.34000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dps |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.082 |
| Number of reflections | 22346 |
| Completeness [%] | 99.0 |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | HEPES 0.1 M, IN A PH RANGE BETWEEN 7.0-7.8. AMMONIUM SULFATE IN A RANGE BETWEEN 1.5-2.0 M |
