1UVB
Lipid Binding in Rice Nonspecific Lipid Transfer Protein-1 Complexes from Oryza sativa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 160 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-15 |
| Detector | MSC |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 48.030, 72.700, 49.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mzm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.590 |
| Number of reflections | 5340 | |
| <I/σ(I)> | 25 | 3 |
| Completeness [%] | 91.7 | 82 |
| Redundancy | 5.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | POLYETHYLENE GLYCOL 600, pH 5.60 |
