1UVA
Lipid Binding in Rice Nonspecific Lipid Transfer Protein-1 Complexes from Oryza sativa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 160 |
| Collection date | 2003-07-15 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.650, 74.490, 49.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.080 - 2.500 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mzm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.064 | 0.300 |
| Number of reflections | 3154 | |
| <I/σ(I)> | 5.6 | 2 |
| Completeness [%] | 93.5 | 92 |
| Redundancy | 4.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | POLYETHYLENE GLYCOL 600, pH 5.60 |
