1UV7
periplasmic domain of EpsM from Vibrio cholerae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-12 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9791,0.9795,0.9686 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 52.879, 52.879, 112.484 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| Rwork | 0.196 |
| R-free | 0.24200 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.302 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.290 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.047 | 0.627 |
| Number of reflections | 20676 | |
| <I/σ(I)> | 18 | 2.6 |
| Completeness [%] | 99.7 | 93.4 |
| Redundancy | 6.61 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 277 | 6-12MG/ML PROTEIN IN 20MM TRIS PH8, 150MM NACL, 1MM TCEP; RESERVOIR: 2.4-3.0M SODIUM MALONATE, 100MM TRIS PH~8; CRYSTALLISATION: 1.5MKL PROTEIN + 1.5MKL RESERVIOR, 4C, pH 8.00 |
