1UV7
periplasmic domain of EpsM from Vibrio cholerae
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-12 |
Detector | ADSC CCD |
Wavelength(s) | 0.9791,0.9795,0.9686 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 52.879, 52.879, 112.484 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
Rwork | 0.196 |
R-free | 0.24200 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 2.302 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.290 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.047 | 0.627 |
Number of reflections | 20676 | |
<I/σ(I)> | 18 | 2.6 |
Completeness [%] | 99.7 | 93.4 |
Redundancy | 6.61 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 277 | 6-12MG/ML PROTEIN IN 20MM TRIS PH8, 150MM NACL, 1MM TCEP; RESERVOIR: 2.4-3.0M SODIUM MALONATE, 100MM TRIS PH~8; CRYSTALLISATION: 1.5MKL PROTEIN + 1.5MKL RESERVIOR, 4C, pH 8.00 |