1UV4
Native Bacillus subtilis Arabinanase Arb43A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.628, 46.153, 57.322 |
| Unit cell angles | 90.00, 98.96, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gyd |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.668 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.300 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.071 | 0.208 |
| Number of reflections | 40400 | |
| <I/σ(I)> | 18.2 | 4.3 |
| Completeness [%] | 91.0 | 74.5 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.2M MGCL2, 15% PEG 3350 5MM CACL2, 10 MM TRIS PH 8.0, 25% ETHYLENE GLYCOL |
